This is a repost of my post on the Biochemistry department: tableconvert-com-free-tabular-data-formats-converter Tabular formats When I first came to Madison in 1986 as a postdoc with Biochemistry professor Paul Kaesberg to learn cloning and sequencing, I was surprised when he said that the most important program to work with sequences was the (now defunct GCG) program… Read More »
Today I found a new tool: bioawk that was written by Heng Li who also wrote samtools and bwa. I first discovered it ont this blog: bioawk-basics (Bioinformatics Workbooks) There is also a short tutorial on GitHub: github.com/vsbuffalo/bioawk-tutorial I also found a recent docker image, and in fact there are only 2 images on docker hub: lbmc/bioawk updated 2 months ago,… Read More »
I use grep very often, and I made-up and acronym that made sense to me: Get REgular ExPression But I discovered this YouTube video that gives an accurate historical recounting of its birth and where its name came from. See video below, titled “Where GREP Came From – Computerphile” Summary: this comes from the command g/re/p… Read More »
Even though I have been using computers all my life there are still many aspects of computing that are mysterious to me, and I am sure many others… One of my favorite quotes is “A leads to B leads to C” (“Future’sEnd” – StarTrek:Voyager, Season 3, episodes 50 & 51) and so my last post being… Read More »
Binder 2.0 As just an “end-user” of software for data analysis it is sometimes difficult to just catch up with what’s available, and I end-up discovering new things simply browsing the web looking for something specific, and ending up finding something else… that was the case for Docker a couple years ago. Today (Dec 30,2019)… Read More »
SRA: Sequence Read Archive The Sequence Read Archive (SRA) makes biological sequence data available to the research community to enhance reproducibility and allow for new discoveries by comparing data sets. The SRA stores raw sequencing data and alignment information from high-throughput sequencing platforms, […] However, it is rather difficult to even find the download links… and even… Read More »
I have started a series of tutorials that I am writing from the perspective of a biologist wanting to use a Docker container for a specific application. An easy example could be using EMBOSS, the molecular biology open suite for analysis. The tutorials are online at the Biochemistry department here: Docker tutorials (general page) Docker… Read More »
Deep neural networks are everywhere. They are revolutionizing our day-to-day lives, and this phenomenon no longer needs any introduction or description. Deep learning is especially suitable to find structures in overwhelming amounts of data. Recently, biological data became exactly that – overwhelming, and application of deep learning toolsets to it indeed looks very natural. You… Read More »
Re: pythonhosted.org/omics_pipe Next Gen. data analysis requires many steps, that can be learned one by one, for example running an aligner such as bowtie, tophat or STAR, then handle the SAM/BAM file for subsequent analysis. Once the various steps are understood and if there are new analyzes do be performed routinely, it would be “nice”… Read More »
Re:alternativeto.net What software one uses may be the result of colleague recommendations, using “what others use in the lab,” or haphazardly found options online. Or perhaps it is time to “upgrade” a commercial package and the steep price makes you think twice… The crowdsources software recommendation web site alternativeto.net does just that… just enter the name… Read More »